Capillary Separations Coupled to Electrospray MS:
This will be a one day course covering the current status of
capillary separations (capillary LC, CE and CEC with column i.d.
in the range of 25-500 um) coupled to electrospray MS and tandem
MS and its capabilities to solve real world problems in proteomics,
biochemistry, pharmaceutical, clinical and environmental analysis.
The course covers the fundamentals of electrospray ionization
with an emphasis on low flow electrospray (nanospray) and layer
flow techniques for low flowrate operation with capillary separations.
The criteria for selecting solvents and additives to achieve good
capillary separations and compatibility with electrospray MS will
be covered. Sample preparation methods to improve concentration
detection limits will be reviewed. Operation tricks and tips to
achieve reproducible and sensitive nanospray signals will be covered.
The course will discuss the interpretation of electrospray mass
spectra and CID mass spectra from tandem MS produced by peptides
and substances of pharmaceutical and environmental significance.
Additionally the advantages and disadvantages of capillary separations
coupled with various mass analyzers (e.g. quadrupole, triple quadrupole,
ion trap and time of flight MS) for qualitative and quantitative
analysis will be reviewed. The course will conclude with a discussion
on chip based separation-nanospray approaches for performing LC/MS
and new technology available for on-line capillary MS. Problem
sets given throughout the course will help reinforce the concepts
Introduction into capillary separations and electrospray mass
Electrospray Mass Spectrometry