Advanced Agilent 1100 / 6300 LC/MSD Trap Course (H8982A)

Course Description:

This four-day lecture and hands-on laboratory course covers advanced concepts in electrospray and APCI LC/ ion trap MSn method development, method optimization, instrument operation, qualitative determinations and quantitative analysis. The majority of the class is devoted to hands on instrument operation with laboratory experiments on the 1100 Series LC/MSD Trap that emphasize the topics covered in the lectures. The course is customized towards pharmaceuticals and biomolecules or for environmental applications (see the course calendar below for course dates).

This Course Includes the following:

  • Four days of lecture and hands-on training
  • Hardcopy of course materials
  • Instruction and problem solving sessions by Dr. Robert D. Voyksner
  • No more than four or five students per instrument
Outline:

  • Advanced ion formation processes in electrospray and APCI
  • Understanding the ion trap and MSn
  • Advanced Electrospray and APCI LC/ion trap MSn operation
  • Optimizing the LC/MSD TRAP method for your analysis
  • Developing a method for qualitative determinations
  • Interpretation of the qualitative results-Interpretation of MSn CID mass spectra
  • Developing a quantitation MS/MS method using external standards
  • Developing a quantitation MS/MS method using isotopically labeled internal standards
  • Increasing sample throughput
  • Developing and optimization of capillary LC/ ion trap MSn
  • System maintenance and diagnostics
  • Solving problem in proteomics* (Pharmaceuticals and Biomolecules course only)

Required Prerequisites:

  • The Agilent H8966A training course or 1-3 months operation of the 1100 LC/MSD Trap.

Student Profile:

  • The course is designed for the operator who wants to understand the advanced concepts of electrospray and APCI LC/ ion trap MS and ion trap MSn and how to better utilize the 1100 LC/MSD Trap in development of method, optimization of methods and for qualitative and quantitative determinations.

Course Calendar:

    March 23-26, 2009
    October 13-16, 2009
How to register:
  • Click on the register button to register electronically.

Please direct all questions about this course offering to: