Two day course
Instructor: Robert D. Voyksner, Ph.D.
LCMS Limited
Durham, NC
USA

This advanced 2 day course will cover generation and interpretation of Collision Induced Dissociation (CID) mass spectra generated from positive and negative ions formed from the API techniques of electrospray, APCI or APPI. The course will cover formation of ions, variables in CID mass spectra under tandem MS (triple quadrupole, q-TOF, ion trap) and API transport CID and interpretation of both the molecular ion and common molecular adduct ions and the product ion formed from these species after CID. The interpretation of the product ion mass spectra will be broken down into distinctive fragmentation mechanisms to aid the student to develop a systematic approach towards spectral interpretation. The fragmentation processes covered include single bond cleavage, multiple bond cleavage with charge retention and with charge migration, cyclization, ring opening fragmentation, hydrogen rearrangements, and charge remote fragmentation. The fragmentation mechanisms will be applied to both positive and negative ions. Additionally, CID product ion formation from adducts such as [M+Na]+ [M+NH4]+ and [M+F, Cl or Br]- will be covered. Each of the lectures have imbedded practical problems, which will allow a review of the various fragmentation mechanisms on a variety of compound types. The course will finish with a series of real world LC/MS/MS interpretation problems applicable to pharmaceuticals, clinical, industrial, and forensic and biochemical industry that the students will work on in groups and discuss at the end of the class.

The course is designed for the students who have completed a LC/MS Short Course or who have some working knowledge of LC/MS and want to improve their qualitative interpretation skills for problem solving in LC/MS. The outline for the course is given below: